OR22-1 Temporal And Spatial Cellular Heterogeneity Of Hepatocyte Metabolism
نویسندگان
چکیده
Abstract Gene expression plays a pivotal role in the long-term regulation of gluconeogenesis and lipogenesis liver. Although most previous studies were analyzed as whole liver, it is known that hepatocytes across liver display specific aspects function zonation dependent manner. Using combination single-cell RNA sequencing (scRNA-seq) single molecule Fluorescence In Situ Hybridization (smFISH) technologies, we examined several novel molecular cellular normal hepatocyte metabolic gene during transitions from fed to fasted, subsequent starvation states male C57BL/6J mice. Targeted scRNA-seq was used quantitively analyze temporal spatial differences gluconeogenic lipogenic genes following post-prandial time course. Initially, state (such Fasn) highly expressed both periportal (PP) pericentral (PC) rapidly turned off mouse entered fasted state. contrast, Pck1) subset PP fully As became activated primarily hepatocytes. However, enters PC also increase their genes. Analyses by smFISH not only confirmed data but applicable determine context native tissue well analyzing transcriptional at resolution. state, few transcription sites (TS), time-course, over TS expanded outwards On other hand, detected. summary, these suggest dynamic can vary substantially depending upon states. Understanding physiology will provide basis for which more precisely dysregulation occurs insulin resistance. Presentation: Monday, June 13, 2022 11:00 a.m. - 11:15
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ژورنال
عنوان ژورنال: Journal of the Endocrine Society
سال: 2022
ISSN: ['2472-1972']
DOI: https://doi.org/10.1210/jendso/bvac150.946